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. 2001 Nov;75(22):10582–10592. doi: 10.1128/JVI.75.22.10582-10592.2001

FIG. 3.

FIG. 3

Analysis of plasmid DNA in Raji transformants established with pHEBo-1.1 and its derivatives bearing mutations within single and paired EBNA-1 binding sites. Low-molecular-weight DNA isolated from Raji cells (lane 1) or hygromycin-resistant Raji clones transformed by pHEBo-1.1 (lanes 2 and 3), pHEBo-1.1(dpm1) (lanes 4 and 5), pHEBo-1.1(dpm2) (lanes 6 and 7), pHEBo-1.1(dpm3) (lane 8), pHEBo-1.1(dpm4) (lanes 9 and 10), pHEBo-1.1(dpm1+2) (lane 11), or pHEBo-1.1(dpm3+4) (lanes 12 and 13) was analyzed by Southern blotting with radiolabeled pHEBo-1.1 DNA. The samples were either digested with SacII (A) or analyzed without prior restriction enzyme digestion (B). The positions of covalently closed circular (fI), nicked circular (fII), and linear (fIII) pHEBo-1.1 DNA are indicated on the right. Also indicated are the positions of Raji EBV DNA containing oriP that was generated by SacII digestion (A) or mechanical shearing during isolation of low-molecular-weight DNA (B).