TABLE 1.
Reagents and tools table.
| Reagent/resource | Reference or source | Identifier or catalog number |
|---|---|---|
| Experimental models | ||
| C57BL/6J (M. musculus) | Jackson Lab | B6.129P2Gpr37tm1Dgen/J |
| BoyJ (CD45.1) mice | Jackson Lab | B6.SJL‐Ptprca Pepcb/BoyJ |
| Recombinant DNA | ||
| ‐ | ||
| Antibodies | ||
| Biotin Mouse Lineage Panel | BD Biosciences | Cat # 559971 |
| Streptavidin‐APC‐Cy7 | BD Biosciences | Cat # 554063 |
| Anti‐CD11b | eBioscience | clone M1/70 |
| Anti‐B220 | eBioscience | clone RA3‐6B2 |
| Anti‐CD5 | eBioscience | clone 53–7.3 |
| Anti‐Gr‐1 | eBioscience | clone RB6‐8C5 |
| Anti‐Ter119 | eBioscience | 13–5921‐82 |
| Anti‐CD8a | eBioscience | clone 53–6.7 |
| Anti‐Sca‐1 | eBioscience | clone D7 |
| Anti‐c‐Kit | eBioscience | clone 2B8 |
| Anti‐CD34 | eBioscience | clone RAM34 |
| Anti‐Flk2 | eBioscience | clone A2F10 |
| Streptavidin, AF 700 | ThermoFisher | Cat # S21383 |
| CD34‐FITC, clone RAM34 | eBioscience | Cat # 11‐0341‐82 |
| Flt‐3 PE | eBioscience | Cat # 12‐1351‐82 |
| C‐KIT–APC | eBioscience | Cat # 17‐1171‐82 |
| Sca‐1 PE‐Cy7 | eBioscience | Cat # 25‐5981‐82 |
| cKit–APC‐Cy7 | eBioscience | Cat # A15423 |
| CD45.2–FITC | Biolegend | Cat # 109806 |
| CD45.1–PE | eBioscience | Cat # 12‐0453‐82 |
| Anti‐CD3e–PE‐Cy7 | Biolegend | Cat # 100320 |
| Anti‐LAMP2 antibody, GL2A7 | Abcam | Cat # ab13524 |
| Anti LC3A/B | Cell Signalling | Cat # 4108 |
| AcH3K27 | Millipore | Cat # 07–360 |
| AcH4K16 | Abcam | Cat # ab13524 |
| Anti‐rat dylight488‐conjugated antibody | Jackson ImmunoResearch | Cat # 112‐485‐071 |
| Anti‐Rabbit Alexa Fluor 555 | Invitrogen | Cat # A32794 |
| Oligonucleotides and other sequence‐based reagents | ||
| ‐ | ||
| Chemicals, enzymes and other reagents | ||
| 96‐well round bottom plates (TPP Tissue culture plates) | Midwest Scientific | TP92097 |
| 96‐ well flat bottom plates | Suspension Sarstedt | Cat # 83.3924 |
| Antifade Mounting Media | VECTASHIELD Laboratories | Cat # H‐1000‐10 |
| Carboxy SNARF‐1 AM | ThermoFisher | Cat # C1272 |
| Heat‐Inactivated Fetal Bovine Serum | GIBCO | Cat# 10438‐026 |
| DCF‐DA | Invitrogen | Cat # D399 |
| Click‐iT Plus EdU AF488 Assay Kit | Thermo Fisher | Cat # C10420 |
| SMART‐Seq v4 Ultra Low Input RNA kit | Clontech | Catalog # 634892 |
| Nextera XT DNA Library Preparation kit | Illumina | Catalog # FC‐131‐1096 |
| Donkey Serum | Sigma | Cat # 566460 |
| Iscove modified Dulbecco medium (IMDM | Cellgro, | Cat#21‐020‐CV |
| Penicillin/Streptomycin | Corning | Cat# 30‐002‐CI |
| Histopaque 1083 | Sigma | Cat# 10831 |
| DAPI | ThermoFisher | Cat # D1306 |
| Nigericin | Sigma | Cat # N7143 |
| NaCl | Sigma | Cat # S7653 |
| KCl | Sigma | Cat # P5405 |
| HCl | Sigma‐Merck | Cat # H1758 |
| NaOH | Sigma | Cat # S8045 |
| 7AAD | Life Technologies | Cat # A1310 |
| Cytokines | ||
| Mouse stem cell factor | Prospec | CYT‐275 |
| Murine thrombopoietin | Prospec | CYT‐346 |
| Human recombinant G‐CSF | Amgen | Neupogen 300 μg/ml |
| Human Recombinant TPO | Stemcell Technologies | 78,210 |
| Human Recombinant IL‐3 | Stemcell Technologies | 78,040 |
| Human Recombinant IL‐6 | Stemcell Technologies | 78,050 |
| Human Recombinant SCF | Stemcell Technologies | 78,062 |
| Human Recombinant Flt3/Flk‐2 Ligand | Stemcell Technologies | 78,009 |
| Software | ||
| GraphPad Prism8.0 | https://www.graphpad.com | |