FIG. 3.

Inhibition of VSV release by MG132. (A) Titers of VSV present in the supernatant of cells infected in the absence (−) or presence (+) of MG132 (50 μM). VSV titers and standard deviations represent averages of six independent experiments. (B) Time course analysis of VSV replication. Viral titers were determined from supernatant samples harvested at the indicated time points from cells infected in the presence or absence of MG132 (50 μM). MG132 was added to VSV-infected cells at 5.5 h postinfection (arrowhead). (C) Immunoprecipitation of radiolabeled VSV M protein from cell extracts (cells, lanes 1 and 2) and supernatants (media, lanes 1 and 2) of cells infected in the absence (−) or presence (+) of MG132 (50 μM).