Table 1.
Information about primary antibodies used.
| Antibody | Species Raised | Isotype | Application, dilution | Catalog number | Source |
|---|---|---|---|---|---|
| TH | Rabbit polyclonal | IgG | WB, 1:1000 IHC/IF, 1:500 |
T8700 | Sigma-Aldrich |
| TH | Mouse monoclonal | IgG1κ | IHC/IF, 1:200 | MAB318 | Millipore |
| Phospho-Akt (Ser 473) | Rabbit monoclonal | IgG | WB, 1:1000 | 4060S | CST |
| Akt | Rabbit monoclonal | IgG | WB, 1:1000 | 4691S | CST |
| Phospho-GSK-3β (Ser 9) | Rabbit monoclonal | IgG | WB, 1:1000 | 9322S | CST |
| GSK-3β | Rabbit monoclonal | IgG | WB, 1:1000 | 12456S | CST |
| CDK5 | Rabbit monoclonal | IgG | WB, 1:1000 | 14145S | CST |
| Nrf2 | Rabbit polyclonal | IgG | WB, 1:1000 IF, 1:200 |
SAB4501984 | Sigma-Aldrich |
| MEF2D | Mouse monoclonal | IgG3 | WB, 1:500 IF, 1:200 |
sc-271153 | Santa Cruz |
| SIRT1 | Mouse monoclonal | IgG1 | WB, 1:1000 | 8469S | CST |
| PGC-1α | Rabbit Polyclonal | IgG | WB, 1:500 IF, 1:50 |
PA5-38022 | Invitrogen |
| PGC-1α | Rabbit polyclonal | IgG | IF, 1:250 | HPA063136 | Atlas |
| TFAM | Rabbit monoclonal | IgG | WB, 1:1000 | 8076S | CST |
| HO-1 | Rabbit monoclonal | IgG | WB, 1:1000 | 43966S | CST |
| 8-OHdG | Rabbit polyclonal | IgG | IF, 1:250 | orb16710 | Biorbyt |
| 4-HNE | Mouse monoclonal | IgG | IF, 1:200 | SMC-511D-A594 | StressMarq |
| 3-NT | Mouse monoclonal | IgG | IF, 1:200 | SMC-154D-STR | StressMarq |
| Lamin A/C | Mouse monoclonal | IgG2a | WB, 1:1000 | 4777S | CST |
| β-actin | Rabbit monoclonal | IgG | WB, 1:1000 | 4970S | CST |
Abbreviations: WB: Western blot; IF: Immunofluorescence; IHC: Immunohistochemistry; ICC: Immunocytochemistry; CST: Cell Signaling Technology.