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. 2024 Oct 9;15(10):734. doi: 10.1038/s41419-024-07089-4

Fig. 3. HIF-1α activates DTL transcription under hypoxia.

Fig. 3

A, B HCC cells were transfected with HIF-1α-siRNA or treated with KC7F2 (40 μM) under hypoxia (1% O2). The mRNA and protein expression of DTL and HIF-1α were determined using qRT-PCR and a western blot assay. C Dual-luciferase reporter assays were performed in HEK-293T cells with HIF-1α overexpression or hypoxia following KC7F2 treatment (40 μM). D Sequences of the predicted HRE in the DTL promoter. E, F Chromatin immunoprecipitation (ChIP) assays in Huh-7 and HCCLM-3 cells to identify the binding of HIF-1α to the DTL promoter under hypoxia or after treatment with CoCl2. G Schematic diagram of the DTL promoter and three mutant constructs. H Dual-luciferase reporter assays for the mutant HRE sequences in HEK-293T cells under hypoxia. I, J Dual-luciferase reporter assays for the mutant HRE sequences in HEK-293T cells following siHIF-1α and KC7F2 (40 μM) under hypoxia. *P < 0.05, **P < 0.01, ***P < 0.001, ns no significance.