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. 2024 Oct 9;15(10):734. doi: 10.1038/s41419-024-07089-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A Heatmap of the representative upregulated and downregulated gene after overexpression of DTL in HCCLM-3 cells. B Gene set enrichment analysis (GSEA) of the Notch pathway. C qRT-PCR results of Notch1 and EMT-related genes mRNA expression in DTL overexpressing or knockdown HCC cells. D Western blot results of Notch1 and EMT-related genes protein expression in DTL overexpressing or knockdown HCC cells. E Immunofluorescence results of Notch1 and N-cadherin expression in DTL overexpressing or knockdown HCCLM-3 cells. F, G qRT-PCR and western blot detected the knockdown efficiency of Notch1 by siRNA. H, I The functional role of Notch1 in DTL-induced proliferation of HCC cells was detected by Cell Counting Kit-8 (CCK-8) and clone formation assays. J, K The functional role of Notch1 in DTL-induced metastasis of Huh-7 cells was detected by wound-healing and transwell assays. L CCK-8 assay evaluating Huh-7 cells viability under DTL overexpression or hypoxia with Notch1 knockdown and sorafenib (3 μM) treatment. *P < 0.05, **P < 0.01, ***P < 0.001, ns no significance.