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. 2024 Oct 9;10:431. doi: 10.1038/s41420-024-02202-0

Fig. 2. Exosome-circMETTL3 promotes macrophage M1 polarization.

Fig. 2

A The level of circMETTL3 was detected using the Fish assay in THP-1 cells pretreated with PMA and subsequently added with exosomes from health and sHLH pediatric plasma for 24 hours. Error bars represent mean ± SD from n = 3 independent experiments. B Western blotting was used to examine the expression of iNOS and CD86 in THP-1 cells pretreated with PMA after stimulation with the addition of healthy and HLH exosomes for 6 hours and then treated with or without LPS for 24 hours. C–E Multi-Cytokine Kit Analysis the cytokines level (IL-6, GM-CSF, IL-1β) of PMA-differentiated THP-1 cells after health and HLH plasm exosome stimulation with or without LPS/IFN-γ treatment. F–J The cytokines level (IL-6, GM-CSF, IL-1β,TNF-ɑ, IL-10) of PMA- differentiated THP-1 cells after knock down circMETTL3 with or without LPS/IFN-γ treatment. (In C–J, error bars represent mean ± SD from n = 3 independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s. not significant).