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. 2024 Sep 26;12:1450838. doi: 10.3389/fbioe.2024.1450838

FIGURE 1.

FIGURE 1

Workflow. Once the hydrogel was produced, three main preliminary printing tests were carried out to define the best ink composition (A), the best crosslinking method (B), and if it was possible to print a shape with the investigated hydrogels (C). The next step consisted in combining the hydrogel with cells (MS5) (D). The mixtures were analyzed plane (not printed) and after the 3D cell printing process. Cell morphologywas assessed, and cell viability was tested by using both the live/dead staining and the ATP assay.