TABLE 3.

Oligonucleotide primers used during site directed mutagenesis.

Oligonucleotide primers used for PCR amplification
Mutation (A)	Primer	Sequence (5′-3′)
Q42	Q42A_fwd_3.2	GTA TGC GTC ACG GCC gcg CAT CGT CAG ATG TTG
Q42	Q42A_rev_3.2	CAA CAT CTG ACG ATG cgc GGC CGT GAC GCA TAC
Q69	Q69A_fwd_3.2	TCT GTG CGT GTC CTT gcg GGA CTG GAG CCT GTT
Q69	Q69A_rev_3.2	AAC AGG CTC CAG TCC cgc AAG GAC ACG CAC AGA
E135	E135A_fwd_3.2	CTT TCT CCG TTT CCA gcg GAA ATG AAC CGT CAA
E135	E135A_rev_3.2	TTG ACG GTT CAT TTC cgc TGG AAA CGG AGA AAG
H241	H241_fwd_2.3	ATC GTA TAT CCG GTT gcc CCA AGT CCG GCT GTA
H241	H241_rev_2.3	TAC AGC CGG ACT TGG ggc AAC CGG ATA TAC GAT