TABLE 5.

Comparison of the kinetic data obtained for the forward reaction (fwd) catalyzed by the PaMnA wild-type enzyme and allosteric variants of PaMnaA with those from Bacillus anthracis (Velloso et al., 2008), Neisseria meningitidis (Zhang et al., 2016), and Staphylococcus aureus (Mann et al., 2016) wild-type UDP-GlcNAc 2-epimerases (fwd).

Paenibacillus alvei				Bacillus anthracis				Neisseria meningitidis				Staphylococcus aureus
This study				Velloso et al., 2008				Zhang et al., 2016				Mann et al., 2016
	K M_fwd [mM]	k cat_fwd [s-1]	k cat/K M [s-1mM-1]		K M_fwd [mM]	k cat_fwd [s-1]	k cat/K M [s-1mM-1]		K M_fwd [mM]	k cat_fwd [s-1]	k cat/K M [ s-1mM-1]		K M_fwd [mM]	k cat_fwd [s-1]	k cat/K M [ s-1mM-1]
Wild-type	3.91	33.44	8.56	Wild-type	2.20	7.90	3.60	Wild-type	3.60	31.00	8.60	Wild-type	0.41	-	-
Q42A	1.61	0.53	0.03	Q43A	17.20	1.20	0.07
Q69A	8.92	37.92	4.25	Q70A	11.10	2.06	0.19
H241A	3.27	0.53	0.16	H242A	10.40	0.22	0.021