Figure 1. GH188 analysis.

A ) SSN for GH188 sequences. The corresponding residue at position L170 with respect to characterized GH188 sequence are differentially colored. The SSN cluster containing the structurally characterized GH188 is circled in green, the Y160 SSN cluster in blue, and Y158 cluster in purple. B ) Comparison of sulfonate binding site residues for the GH188 crystal structure (green; PDB ID 8QC2) (Kaur et al., 2023) and the AlphaFold models for representative Y160 (blue) and Y158 (purple) sequences. NAD ⁺ and SQ are shown in yellow and are from the GH188 crystal structure. C ) Comparison of the loop region surrounding the L170, Y160, and Y158 side chain and D ) the dimer interface involving that loop region. Nitrogens are shown in blue, oxygens in red, sulfur is dark yellow, and phosphorus in orange. E ) Genomic neighborhood surrounding GH188 sequences that correspond with Y160 and Y158, and the Sulfo-EMP pathway (Kaur et al., 2023). Plus signs represent conserved genes between Y160 and Y158 gene clusters and stars represent conserved genes between Y160, Y158 and the Sulfo-EMP pathway gene clusters.