Figure 6.

Effects of propofol on the molecular markers of cell apoptosis and cell cycle progression in 4T1 cells. (A) The protein expression levels of apoptosis antagonist Bcl-xL, apoptosis agonist Bax, and apoptosis activator cleaved caspase 3 were assessed after treatment with 100 µg/ml propofol for 24 h in 4T1 cells. Quantification of the protein levels is presented. (B) The protein expression levels of cell cycle makers CCNE2, p-CDC6 and CDC6 were analyzed in 4T1 cells following treatment with 100 µg/ml propofol for 24 h. Quantification of the protein levels is presented. (C) Immunofluorescence images of CCNE2 and p-CDC6 in 4T1 cells after treatment with 100 µg/ml propofol for 24 h. (D) The relative expression of CCNE2 and CDC6 mRNA in 4T1 cells after treatment with 100 µg/ml propofol for 24 h. Statistical analysis was performed using the Student's t-test. Data are presented as the mean ± SD (n=4). *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001. ns, no significance; ctrl, control; Bcl-xL, B-cell lymphoma-extra large; CCNE2, cyclin E2; p-CDC6, phosphorylated cell division cycle 6.