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. 2024 Oct 9;7(12):e202402795. doi: 10.26508/lsa.202402795

Figure 2. The spatiotemporal expression of naa80 mRNA by whole-mount in situ hybridization and RT-qPCR.

Figure 2.

(A, B, B′, C, C′, D, E, F) Whole-mount in situ hybridization was performed at different developmental stages of zebrafish embryos at 1 (A), 24 (B, B′), 48 (C, C′) and 72 (D) hpf. RT-qPCR at different embryonic stages (E) and adult tissues (F). For (A, B, B′, C′), dorsal view and anterior to the left. For (C, D), lateral view and anterior to the left. Fb, forebrain. Mb, midbrain. Hb, hindbrain. Pf, pectoral fin bud. Scale bar = 0.3 mm. For (E, F), each timepoint included biological triplicates as well as technical triplicates. (E, F) The expression levels were first normalized to the 18S housekeeping gene and the expression levels were compared with 18 hpf embryos (E) or compared with skin (F). Data shown are mean ± SD and mean values were shown on each bar.