FIG. 3.

Expression of HSV-2 VP22 begins 4 to 6 h after infection. Vero cells were infected with HSV-2 at an MOI of 5. Infected cells were incubated with [³⁵S]methionine-cysteine for the times indicated. After incubation with the label, cells were lysed in RIPA buffer, and equal volumes were immunoprecipitated with 22-3. VP22 was detected by Western analysis by using 22-3, and ³⁵S was detected by autoradiography. The band labeled with an asterisk is an artifact observed during immunoprecipitation.