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. 2024 Oct 10;14(4):e12448. doi: 10.1002/pul2.12448

Figure 4.

Figure 4

Endothelial depletion of PPARγ reduces ETV2, PECAM1, and VE‐Cad expression and increases levels of EndoMT markers in mouse lungs. (a, b) HPAECs were treated with scrambled (SCR) or PPARγ (20 nM) siRNAs (a, b) or ETV2 (20 nM) siRNAs (c) for 6 h, washed with fresh medium, then incubated for an additional 72 h. Each bar represents mean ± SE PPARγ or ETV2 level relative to GAPDH expressed as fold‐change vs cells treated with SCR. n = 3/group, *p < 0.05 vs SCR. (d–f) Whole lungs were collected from littermate control (LitCon) or endothelial‐targeted PPARγ knockout (ePPARγKO) mice. Lung levels of PPARγ (d), ETV2 (e), or EndoMT and EC markers (f) mRNA levels were measured using qRT‐PCR in littermate control (LitCon) or endothelial‐targeted PPARγ knockout (ePPARγKO) mice and expressed relative to GAPDH mRNA *p < 0.05 vs LitCon, n = 4/group.