Fig. 2.

NCLX inhibition reduces the magnitude of FCCP-induced cytosolic Ca²⁺ surge in WT MEFs

(A) Real-time tracing of Cal-520 fluorescence normalized by the baseline (t = 0 min) for WT MEFs pre-treated with or without CGP-37157 (NCLX inhibitor), following the addition of FCCP at 3 min timepoint (N = 4 experiments, 60 ≤ cells traced per N). (B) AUC analysis of cytosolic Ca²⁺ measurements in (A) from 3 to 10 min in WT MEFs (N = 4 experiments). (C) Real-time reading of percentage decrease in calcein fluorescence in WT MEFs after the addition of ionomycin for 300 s (black line, positive control) or WT MEFs treated with FCCP for the first 300 s, followed by ionomycin treatment (red line) (N = 3 experiments). Data are presented as mean ± SEM. Statistical analyses: (B) Unpaired parametric Student’s t-test between groups with and without CGP-37157 pre-treatment. **p < 0.01