FIG. 8.
PFGE analysis of packaged amplicon DNA. BHK cell monolayers were transfected with pSA1 and superinfected with either wt HSV-1 or KUL25NS. Nuclear and cytoplasmic DNase-resistant DNAs were prepared and analyzed by PFGE as described in the legend to Fig. 5, except that the probe was 32P-labeled pAT153. The markers (M) are a 5 kbp ladder (Bio-Rad), and the positions of the 5- and 70-kbp fragments are shown. The blot was reprobed with labeled pGX153, and the position of unit-length HSV-1 genomes (152 kbp) is indicated.