Table 1.

Primers used in this study.

	Sequences	Target	Purpose	Ref.
Pan-strongylid
PCR
NC1	5′–ACGTCTGGTTCAGGGTTGTT–3′	nuclear ITS2 (nematodes)	Identification of A. cantonensis in rodent organs	This study [15, 16]
NC2	5′–TTAGTTTCTTTTCCTCCGCT–3′
Sequencing	5′–GTGCGTTTTGTGCGTTTAC–3′
NC internal
Worm identification
AngiCOI_forward	5′–TTTTTTGGGCATCCTGAGGTTTAT–3′	cox1 (Angiostrongylus spp.)	Taxonomic assessment of isolated worms	[13, 36]
AngiCOI_reverse	5′–CGAGGATAACCATGTAAACCAGC–3′
Rattus identification
For-cytB-Rattus	5′–CTCCCATGAGGACAAATATC–3′	cytb (rodents)	Confirmation of rodent species by RFLP	This study
Rev-cytB-Rattus	5′–ATGGGTGTTCTACTGGTTG–3′