FIG. 3.

Cell surface expression of CCR5 mutants. QT6 quail cells were transiently transfected with huCCR5 or huCCR5 mutants by the standard calcium phosphate method, and after overnight expression, cells were detached with ice-cold 1 mM EDTA-PBS and analyzed by FACS using a monoclonal antibody to CCR5 (2D7) directly conjugated to phycoerythrin. Histograms are labeled with mean channel fluorescence (MCF). Cells expressing CCR5 are shown with a bold line, while cells transfected with vector alone are shown with a thin line.