Fig. 5.

(A) Response of the calcium sensor after spiking the serum with Ca²⁺. (B) Nernstian behavior of the sensor to two-point calibration in artificial serum and accuracy of the calcium sensor to measurement of calcium in serum. (C) Emf response of the calcium sensor to the insertion of the sensor in calibration solutions and serum. (D) Emf shift of the IL-based reference electrodes after electrode transfer from artificial serum to serum. (E) Similar shift in the emf response of the IL-based reference electrode after successive insertion of electrode in artificial serum with different Ca²⁺ concentration and serum. (F) Shift in the emf response of the Il-based reference electrode after transfer of the electrode from artificial serum to serum in three different adult bovine serums. (G) Nernstian behavior of the sensor in response to two-point calibration in artificial serum, as well as measured calcium levels before and after correcting for IL-based shift. (H) Emf response of the calcium sensor to the insertion of the sensor in calibration solutions and serum versus IL-based reference electrode.