(A) Representative nuclei and MN (white arrows) colocalized with cGAS, γH2AX, neither, or both proteins. Original magnification, ×20. (B) Mean MN numbers per nucleus. (C) Percentages of MN colocalized with cGAS relative to total number of MN. (D) Percentages of MN colocalized with γH2AX relative to total number of MN. Error bars represent the SD from 3 independent experiments. Statistical significance was assessed with 2-way ANOVA with Tukey’s multiple-comparison test. NS, non-significant. *P < 0.05; **P < 0.01; ****P < 0.0001.