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. 2024 Aug 20;9(19):e179417. doi: 10.1172/jci.insight.179417

Figure 1. Bank1 deficiency improves major lupus phenotypes in TLR7.tg6 and in IMQ-treated mice.

Figure 1

Serum levels of antibodies were quantified by ELISA in the TLR7.tg6 model (32 weeks of age), and in the IMQ-induced model (20 weeks of age). (A) Serum levels of IgG antidsDNA antibodies in optical density (OD) at 1:1,000 dilution. Total mice analyzed: WT (n = 14), T7 (n = 16), T7.B1–/– (n = 16); and WT (n = 16), WT + IMQ (n = 19), B1–/– (n = 16), B1–/– + IMQ (n = 19). (B) Serum levels of anti-Sm antibodies (U/mL) at 1:100 dilutions. Total mice analyzed: WT (n = 12), T7 (n = 15), T7.B1–/– (n = 16); and WT (n = 10), WT + IMQ (n = 14), B1–/– (n = 10), B1–/– + IMQ (n = 13). (C) Serum levels of total IgG (μg/mL) at 1:100,000 dilutions. Total mice analyzed: WT (n = 25), T7 (n = 29), T7.B1–/– (n = 29); and WT (n = 10), WT + IMQ (n = 20), B1–/– (n = 10), B1–/– + IMQ (n = 22). (D) Serum levels of IgG2c (μg/mL) at 1:10,000 dilution. Total mice analyzed: WT (n = 15), T7 (n = 15), T7.B1–/– (n = 17); and WT (n = 10), WT + IMQ (n = 19), B1–/– (n = 9), B1–/– + IMQ (n = 22). (E) Serum levels of IgM in optical density (OD) at 1:5,000 dilution. Total mice analyzed: WT (n = 9), T7 (n = 13), T7.B1–/– (n = 12); and WT (n = 7), WT + IMQ (n = 7), B1–/– (n = 8), B1–/– + IMQ (n = 8). Each point represents 1 individual mouse. Data are shown as mean ± SEM. Mann-Whitney U test with Welch’s correction was used to test statistical significance.

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