FIG. 4.

Infection of hPrMC and mature hMC with pseudotyped CAT reporter viruses. (a) Purified hPrMC (4 weeks old) were infected with pseudotyped single-round viruses carrying HIV-1 (ADA, YU2, HXBc2, 89.6, ELI, and ΔKS) and SIV mac316 envelope glycoproteins. (b) hMC (9 weeks old) were infected with the same viruses (except for 89.6, SIVmac316, and ELI) and with VSV G. The reporter gene activity (CAT) in lysates of infected cells is shown 5 days after infection. ΔKS is a nonfunctional HIV-1 envelope and was used to determine nonspecific entry. Results are the means and standard deviations of triplicates from an experiment representative of the four experiments performed.