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The qRT-PCR validation of microarray data. The data represent the fold change in gene expression of 6-month-old mice treated with GeO2 compared to 6-month-old controls given normal chow. The qRT-PCR analyses for Atp5a1, Atp5e, Cox5a, Sdhb, and Sdhc. The qRT-PCR results were in agreement with the microarray findings (light gray bar) that the expression of these mitochondrial function-associated genes was significantly decreased in the cochleae of germanium-treated mice (dark gray bar).
