Figure 2. Plotting of PC1 and PC2 of the principal component analysis (PCA).
The PCA was generated with nuclear DNA data (N = 6,682,591 SNPs (single nucleotide polymorphisms)) from 11 historical butterfly specimens (4 G. xerces and 7 G. lygdamus), a modern G. lygdamus from Canada (RVcoll10-B005) and a modern G. alexis reference genome. The PCA shows a clear separation of both historical species and the reference in the first PC (explaining 52.81% of the variance), and separation of G. xerces and G. lygdamus by the second PC (explaining 6.09% of the variance), supporting they are separated lineages.