Serum LH (a), FSH (b), and testosterone (c), levels in the control, diabetes, early and late insulin, CM, and DMEM-treated diabetic groups. Data were analyzed by one-way ANOVA (Tukey's HSD test) and presented as mean ± SEM (n = 7). ∗P < 0.05, ∗∗P < 0.01, ∗ ∗ ∗P < 0.001, ∗ ∗ ∗ ∗P < 0.0001 vs. the control group; #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 vs. the diabetic group; and +P < 0.05, ++P < 0.01, +++P < 0.001 vs. the D-DME group. D-CME, diabetic group receiving early WJMSCs-CM; D-CME, diabetic group receiving early WJMSCs-CM; D-CML, diabetic group receiving late WJMSCs-CM; D-INSE, diabetic group receiving early insulin; D-INSL, diabetic group receiving late insulin; D-DME, diabetic group receiving early DMEM; D-DML, diabetic group receiving late DMEM; early, treatment was started immediately after diabetes induction; Late, treatment was started 30 days after diabetes induction.