Figure 3.

Quantitative analysis of EGFP-expressing cell types following injection of nine serotype AAV vectors into the marmoset cerebral cortex

(A) Representative fluorescent images of the cortical sections immunostained for GFP (green), NeuN, GFAP, Olig2, and Iba1 (magenta) 4 weeks after injection of AAV5 or AAVrh10. Scale bar, 100 μm. (B–E) Graphs showing the ratio of GFP⁺ neurons (B), GFP⁺ oligodendrocytes (C), GFP⁺ astrocytes (D), and GFP⁺ microglia (E) to total GFP⁺ cells. The dotted lines in the graphs are ratios of respective cell types to total cells present in the marmoset cortex (see Figure S3). Error bars indicate SEM, and the dots in the graph indicate the respective values for each of the individual marmosets. The asterisks indicate a statistically significant difference between the AAV5 vector and AAVrh10. ∗p < 0.05 by a Kruskal-Wallis test with Dunn’s post hoc test. n.s., not significant.