Figure 5.

Efficient EGFP expression in astrocytes by all nine serotype AAVs with the astrocyte-specific hGFA(ABC1D) promoter

(A) Schema depicting the AAV genome structure. (B) Immunofluorescent EGFP images of the cerebral cortex received injections of respective AAV vectors. Scale bar, 100 μm. (C) Representative image immunostained for EGFP alone (left) and merged image for EGFP and GFAP (right) after injection of the AAV2 vector. Scale bar, 100 μm. (D) Graph showing the percentage of GFAP⁺ astrocytes to total EGFP-expressing cells 4 weeks after injection of the AAV vector as indicated. The dotted line in the graph shows a ratio of astrocytes to total cells present in the marmoset cortex (see Figure S3). Error bars indicate SEM, and dots in the graph indicate the respective values for each of the individual marmosets. n.s., not statistically significant by a Kruskal-Wallis test with Dunn’s post hoc test.