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Colocalization of actin with VP1 of virions (a to d) and pseudocapsids (f to h). Sections of NIH 3T6 cells fixed 20 min p.i. (a to c, f, and g) or 3 h p.i. (d and h) were visualized by confocal microscopy. (e) Control, mock-infected, cell fixed 20 min p.i. Staining was done with the rabbit anti-polyomavirus virion serum followed by the Alexa Fluor 488-goat anti-rabbit IgG antibody (green) and with phalloidin conjugated with rhodamine (red). Bars, 10 μm.
