FIG. 2.

pUL6 content of HSV-1 B-capsids as determined by Western immunoblotting. (Top left) B capsid proteins separated by SDS-polyacrylamide gel electrophoresis and stained with Coomassie blue. (Top right) Same as the gel on the left, except that pUL6 is stained specifically by Western immunoblotting. (Bottom) Purified pUL6 stained by Western immunoblotting on the same gel as that shown in the top right. The pUL6 signal in the bottom panel was employed as a standard curve to determine the amount present in B capsids as shown at the top right.