Fig. 6. Receptor abundance defines cell type- but not BC-specific responses.

a Schematic demonstrating our approach to identifying whether subject-level changes in cytokine response can be explained by subject-level alterations in receptor abundance. b Average IFN-induced STAT1 phosphorylation versus IFNγR1 abundance across subjects for each cell type (n = 23). Induced responses are reported as z-scored delta MFI. All reported correlations are Pearson correlations. c Average IL-2-induced pSTAT5 versus IL2Rɑ abundance across subjects for each cell type. d IL10R abundance across cell types, grouped by subject status (healthy n = 22, BC n = 14). Cell types which feature statistically significant responses between BC and healthy status are listed in red. e IL-10-induced pSTAT3 versus IL10R abundance in classical monocytes, across subjects (n = 36). f IL2Rɑ abundance across cell types, grouped by subject status. g IL2Rβ abundance across cell types, grouped by subject status. Significance was derived using the Mann–Whitney U test, comparing those measurements from healthy donors to those of BC patients. Only cell types with significantly altered receptor abundances across cohorts were included in (f, g). h IL-2-induced pSTAT5 versus IL2Rɑ + IL2Rβ in Tregs for each subject. For all box plots, the center line denotes the median, the box limits denote the upper and lower quartiles, and the whiskers denote the 1.5x interquartile range. *, **, and *** represent p values less than 0.05, 0.005, and 0.0005, respectively.