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. 2024 Sep 10;25(10):4433–4464. doi: 10.1038/s44319-024-00243-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV5 — (A) Top left: Representative histograms showing relative counts of WT hESCs expressing α-fucose residues before and after BMP4 treatment for 24 h. Top right: Quantification of the percent of positive WT hESCs expressing α-fucose residues before and after BMP4 treatment for 24 h. n = 3 clones for each clone n = 2 technical replicates, Bottom left: Representative histograms showing relative counts of FUT1⁺ECs expressing α-fucose residues before and after BMP4 treatment for 24 h. Bottom right: Quantification of the percent of positive FUT1⁺ECs expressing α-fucose residues before and after BMP4 treatment for 24 h. n = 3 clones for each clone n = 2 technical replicates. (B) Left: Western blots of pSMAD1/5/8 and β-actin showing a time-course of Smad1/5/8 activity within FUT1⁺ECs and control ECs after BMP4 stimulation for 24 h. Right: Quantification of the pSMAD1/5/8 intensities. The normalized values are relative to normalized β-actin. n = 2 technical replicates. (C) Left: Western blots of pSMAD1/5/8 and β-actin showing a time-course of Smad1/5/8 activity within silenced FUT1 and siNT cells after BMP4 stimulation for 24 h. Right: Quantification of the pSMAD1/5/8 intensities. The normalized values are relative to normalized β-actin. n = 2 technical replicates. (D) Quantification of HAND1 and HOPX 30 h after FUT1 silencing and 6 h after LDN193189 stimulation in WT, siNT, and siFUT1 hESCs relative to WT hESCs. n = 4 biological replicates. Data information: In (A, D), data are presented as means ± SD. Two-tailed Student’s t-tests **p < 0.01 or non-significance (NS).