Reagents and tools table

Reagent/resource	Reference or source	Identifier or catalog number
Experimental models
E. coli DH5α Chemically Competent	Enzynomics	Cat# CP010
E. coli ROSETTA(DE3)	Novagen	Cat# 70954
Recombinant DNA
pET28a-WT rat β-arrestin-2	This study	N/A
pET28a-β-arrestin 2_1-394	This study	N/A
pET28a-β-arrestin 2_Y64A	This study	N/A
pET28a-β-arrestin 2_L280G	This study	N/A
pET28a-β-arrestin 2_E315A	This study	N/A
pET28b-rat β-arrestin-2 _Cysfree_G6W_D386C	This study	N/A
pET28b-rat β-arrestin-2 _Cysfree_G6W_L280G_D386C	This study	N/A
Oligonucleotides and other sequence-based reagents
PCR primer: Y64A Forward: GTGCCTTCCGCGCTGGCCGAGAAGACCTGGATG	Bionics	N/A
PCR primer: L280G Forward: CACCATAACCCCGCTGGGCAGTGACAACCGAGAGAAG	Bionics	N/A
PCR primer: E315A Forward: GAGGGAGCCAACAAGGCGGTGCTGGGAATCCTAG	Bionics	N/A
PCR primer: G6W Forward: CATATGGGTGAGAAGCCCTGGACCAGGGTCTTCAAG	Bionics	N/A
PCR primer: D386C Forward: CCAACTATGCCACAGACGACTGCATCGTGTTTGAGGAC	Bionics	N/A
Chemicals, enzymes and other reagents
Protease inhibitor cocktail	BioVision	Cat# K272
Deuterium oxide	Cambridge isotope laboratories	Cat# DLM-11-100
Ni-IDA resin	Cytiva	Cat# 17057501
Dimethyl sulfoxide	Duchefa	Cat# D1370
Leupeptin	Goldbio	Cat# L-010
Lysozyme	Goldbio	Cat# L-040
TCEP	Goldbio	Cat# TCEP
IPTG	Goldbio	Cat# I2481C
Kanamycin	Goldbio	Cat# K-120-10N
Chloramphenicol	Duchefa	Cat# C0113.0025
Pepsin column	Life Technologies	Cat# 2313100
DNase I	Roche	Cat# 11284932001
Benzamidine	Sigma-Aldrich	Cat# 12072
Imidazole	Sigma-Aldrich	Cat# I2399
08:0 PI(4,5)P2	Avanti Polar Lipids	Cat# 850185
Zeba Desalt Spin Desalting Columns	Thermo Scientific	Cat# 89890
Bromobimane	MedChemExpress	Cat# HY-100041
V2Rpp	Tufts University Core Facility	N/A
Software
Prism 8.0	Graphpad	graphpad.com
PyMol 2.3	Schrodinger	pymol.org
Proteinlynx Global Server 2.4	Waters	www.waters.com
DynamX 3.0	Waters	www.waters.com