FIG. 1.
Effect of anti-gp120 MAbs on IFN-γ production by CD4 T-cell line DMg26 in response to gp120. An ELISPOT assay was performed to examine IFN-γ production by the gp120-specific T-cell line DMg26 following stimulation with gp120SF2, gp120SF2 complexed with anti-gp120CD4BD MAbs (654-D or 559/64D), gp120SF2 complexed with an anti-C5 MAb (450-D), or with no antigen. Recombinant gp120SF2 was tested at 5 μg/ml and mixed with medium alone or with MAbs used at 10 μg/ml. An autologous EBV-transformed BCL was used as APCs in this particular experiment. Each condition was tested in duplicate, and the results were confirmed in two independent experiments. The mean numbers of spot-forming cells (SFCs) per 105 T cells and the standard deviations are presented on the y axis.