FIG. 5.
Anti-gp120CD4BD/gp120 complexes do not inhibit the presentation of HIV-1 p24 or CMV antigens to CD4 T cells. (A) Proliferative response of p24-specific CD4 T-cell line AC-25 was assessed in the presence of p24 alone or p24 mixed with 654-D, gp120, or gp120/654-D complexes, or in the absence of any antigen. An autologous B-cell line was used as APCs in this experiment. (B) Lymphoproliferative responses of PBMCs from a CMV-seropositive individual were measured to CMV antigens alone, CMV + gp120/654-D complexes, CMV + 654-D, or no antigen. In each of these experiments, anti-gp120CD4BD MAb 654-D (5 μg/ml) was preincubated with gp120SF2 (2 μg/ml) and then mixed with p24 (1 μg/ml) or CMV antigen (diluted 1:10) (A and B, respectively). T-cell responses were measured in [3H]thymidine incorporation assays. The responses to p24 and CMV in the absence or presence of 654-D and the gp120/654-D complex were not significantly different (P ≥ 0.1 by the Mann-Whitney test).