FIG. 2.

Definition of epitope residues crucial for binding to rhesus macaque MHC class II molecules. (a) Relative binding of single-amino-acid-substituted analogs of Hiv Env 248–262 (THGIRPVVSTQLLL) to Mamu-DRB1*0406, normalized to the binding of the unsubstituted peptide (3.3 nM). (b) Relative binding of single-amino-acid-substituted analogs of Hiv Env 482–497 (ELYKYKVVKIEPLGVA) to Mamu-DRB*w201, normalized to the binding of the unsubstituted peptide (4.9 nM). The dashed line denotes a 10-fold reduction in binding compared with that of the unsubstituted peptide.