FIG. 4.
Intracellular staining performed on PBMC of a Mamu-DRB*w201-positive macaque immunized with DNA constructs encoding the entire SIV genome. Plots show events gated through both CD4+ and lymphocyte gates. (a) Three pools containing 15-mer peptides overlapping by 11 amino acids spanning the entire Rev protein sequence of SIVmac239. (b) Peptides from Rev pool A tested individually. Bold type in peptide sequence indicates a peptide which had been predicted to bind Mamu-DRB*w201 in binding assays.