TABLE 3.

SIV-derived overlapping 15-mer peptide binding to Mamu-DRB^*w201 and Mamu-DRB1^*0406

Sequence	Protein	Position	Binding capacity (IC50 [nM])a		Mamu-DR motif
			Mamu-DRB1*0406	Mamu-DRB*w201
GVPAWRNATIPLFCA	Env	31	7	242	+
LIRILQRALFMHFRG	Vpr	61	51	31	+
GFLGFLATAGSAMGA	Env	531	62	–	−
FAKIKNTHTNGVRLL	Pol	561	66	284	+
WDFISTPPLVRLVFN	Pol	621	70	19	+
MEEEKRWIAVPTWRI	Vif	1	88	21	+
RRWRRRWQQLLALAD	Rev	41	–	13	+
NIYRRWIQLGLQKCV	Gag	261	–	33	+
RKRLRLIHLLHQTNP	Rev	11	–	34	+
GTDIKVVPRRKAKII	Pol	1021	–	35	+
YLCLIQKALFMHCKK	Vpx	71	–	36	+
WHKVGKNVYLPPREG	Env	441	–	52	−
KRRRTPKKAKANTSS	Tat	81	–	59	−
EFRQYTAFTLPSVNN	Pol	331	651	61	+
LALADRIYSFPDPPT	Rev	51	–	86	+
EMIKKSEIYVAWVPA	Pol	731	–	98	−
WRRDNRRGLRMAKQN	Vif	171	104	–	−
IVAVHVASGFIEAEV	Pol	841	104	–	+
CAFRQVCHTTVPWPN	Env	611	139	483	−
QFSLWRRPVVTAHIE	Pol	111	143	239	−
WLSTYAVRITWYSKN	Vif	81	165	145	+
TIVLMAVHCMNFKRR	Pol	941	166	–	+
ITTEQEIQFQQSKNS	Pol	971	203	–	−
DCTHLEGKIIIVAVH	Pol	831	227	–	−
ANFPGLAKVLGILA	Vif	201	240	171	+
RIREQANSVETIVLM	Pol	931	259	–	+
VILLRIVIYIVQMLA	Env	701	319	–	+
ILQRLSATLQRIREV	Env	801	340	–	+
SAMGAASLTLTAQSR	Env	541	413	841	+
KGTIMTGDTPINIFG	Pol	181	623	–	−
EVRRAIRGEQLLSCC	Vif	121	629	–	−
INIFGRNLLTALGMS	Pol	191	689	–	+
AILLLSVYGIYCTLY	Env	11	732	–	+
TSRPTAPSSGRGGNY	Gag	121	740	–	−
MHFRGGCIHSRIGQP	Vpr	71	846	–	−
LDRFGLAESLLENKE	Gag	41	893	–	−
LAIQQLQNLAIESIP	Rev	71	927	–	+
FMPKCSKVVVSSCTR	Env	251	–	108	+
PVTIMSGLVFHSQPI	Env	321	–	115	+
HKYQVPSLQYLALKV	Vif	141	–	139	+
DVKRYTTGGTSRNKR	Env	511	–	143	−
CISEADASTPESANL	Tat	21	–	155	+
IVQQQQQLLDVVKRQ	Env	561	–	159	+
NQQAELEAFLMALTD	Pol	681	–	162	−
SLESSNERSSCISEA	Tat	11	–	193	−
ALKHFDPRLLTALGN	Vpr	31	–	210	+
YVPHFKVGWAWWTCS	Vif	41	–	242	−
TMSAEVAELYRLELG	Env	481	–	245	+
GVRLLAHVIQKIGKE	Pol	571	–	248	+
FLIRQLIRLLTWLFS	Env	771	–	250	+
NIIVDSQYVMGIITG	Pol	701	–	251	+
ALGMSLNFPIAKVEP	Pol	201	–	255	−
LTVWGTKNLQTRVTA	Env	581	–	266	+
PRELIFQVWQRSWEY	Vpx	41	–	277	−
RIREVLRTELTYLQY	Env	811	–	309	+
KYHSNVKELVFKFGL	Pol	781	–	381	−
LSRVYQILQPILQRL	Env	791	–	407	+
YHCQFCFLKKGLGIC	Tat	61	–	412	−
SRNKRGVFVLGFLGF	Env	521	–	504	+
HGDTLEGAGELIRIL	Vpr	51	–	571	−
TAHIEGQPVEVLLDT	Pol	121	–	727	+
VVKRQQELLRLTVWG	Env	571	–	778	+
CVKLSPLCITMRCNK	Env	101	–	821	−
RYIYKVLPQGWKGSP	Pol	351	–	825	−
KDGQLEEAPPTNPYN	Pol	251	–	830	−
KEPFQSYVDRFYKSL	Gag	291	–	856	+
ALAPVPIPFAAAQQR	Gag	371	–	953	+
GEGHGAGGWRPGPPP	Vpx	91	–	958	−
TWGTTQCLPDNGDYS	Env	51	–	969	−
ARLMAEALKEALAPV	Gag	361	–	980	+

^aValues represent the concentration of test peptide required to inhibit 50% of the radiolabeled peptide ELYKFKVVKIEPLGVA (Mamu-DRB*w201) and VSTVQCTHGIRPVVSTQLLL (Mamu-DRB1*0406). –, IC₅₀ of >1,000 nM.