Fig. 6.

Macrophage differentiation profile upon incubation in 0.7 % supernatant of platelets cultured on the different surfaces, or in control M1, M2, or normal media. A) Cell viability from day 1 to day 7 measured using resazurin (n = 3). B) Gene expression of key macrophage differentiation genes at day 3 (n = 3), showing fold difference in comparison to normal non-supplemented culture media. C-D) Fluorescence intensity per cell measured from microscopy images (n = 3) using ImageJ, following staining for CD80 (M1 marker) and CD163 (M2 marker) respectively. E) Pro-inflammatory and pro-healing cytokines produced by macrophages over time using LegendPlex™ flow cytometry, with TNF-α, IFN-γ, IL-1β and IP-10 associated with pro-inflammatory phenotype, while IL-4 and IL-10 are considered to be more pro-healing factors (n = 3). All graphs show mean ± SEM. One-way ANOVA with Dunnett's multiple comparison tests against normal media control (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001).