Fig. 7.

BMSCs differentiation profile upon incubation in 0.7 % v/v supernatant of platelets cultured on the different surfaces, or in control osteoblast and normal media. A) Cell viability from day 1 to day 21 measured using resazurin. B) ALP concentration U/mL/cell. C) Color intensity of BMSCs at day 21 after staining with alizarin red (mineralization indicator), Alcian blue (chondrocyte indicator), or oil red O (adipocyte indicator). D) Representative images of BMSCs stained with alizarin red on day 21. All graphs showing mean ± SEM, n = 3. One-way ANOVA with Dunnett's multiple comparison tests against normal media control (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001).