Figure 4.

Comparison of the binding, cleavage activity, and cleavage product formation of WT and Dicer mutants for pre-miR-21, snord37, and blunt pre-miR-21. (A) Comparison of binding across substrates at 120 min. Quantified data from three independent experiments (Figure S3 and Table S1). (B) Characterization of Dicer cleavage product formation; representative data from three independent experiments (Figure S4). Cleavage products were observed for pre-miR-21 and snord37 after a 120 min reaction. Blunt pre-miR-21 showed an increase in the levels of multiple cleavage products of varying sizes based on positions in the gel.