FIG. 6.

Comparative Gag expression in transfected C2C12 cells with p-syngag and p-syngagΔCpG constructs. C2C12 cells were transiently transfected by calcium phosphate precipitation with the indicated plasmids, and cells (A) and cell culture supernatants (B) were harvested 48 h posttransfection. Control C2C12 cells were transfected with the pcDNA3 plasmid. Cell lysates (50 μg of total protein) and sucrose-sedimented proteins from the culture supernatant were separated by SDS–10% PAGE and analyzed by immunoblotting with a p24-specific monoclonal antibody. Arrows at the right indicate the position of the Gag polyprotein. Sizes are indicated in kilodaltons.