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. 2023 Feb 17;12(13):2202590. doi: 10.1002/adhm.202202590

Figure 4.

Figure 4

Characterization and cytotoxicity of YK009‐LNP‐Omicron mRNA. A) Bioanalysis of Omicron mRNA. B) Physicochemical parameters of YK009‐LNP‐Omicron mRNA. Physicochemical property characterization includes particle size, polydispersity index, Zeta potential, and encapsulation efficiency. C) Transmission electron microscopy image (scale bar = 50 nm) and cryo‐electron microscopy image (scale bar = 100 nm) of YK009‐LNP‐Omicron mRNA. D) pK a of YK009‐LNP‐Omicron mRNA was determined by TNS assay (n = 3). E) Cell viability of HEK‐293T, RD, and DC 2.4 cells after transfection with MC3‐LNP, YK009‐LNP, mRNA, MC3‐LNP‐Omicron mRNA, and YK009‐LNP‐Omicron mRNA (mRNA = 1 µg) for 24 h. Significance was calculated by one‐way ANOVA and data are presented as mean ± SEM.