FIG. 1.

Strategy to clone a cDNA capable of supporting HCV replication in a nonpermissive cell line. (A) HCV infection assay performed with HepG2 cells (left panel) and 293EBNA cells (right panel). Infection assays were performed every 2 days (d) up to 14 days. The experiments were repeated three times. A representative result is shown here. In each panel, the bar to the left indicates the predicted position of the PCR product. As a control, β-actin mRNA was detected simultaneously. P, positive control with HCV-positive serum. (B) Detection of CD81 mRNA by RT-PCR. (C) Flowchart of the cloning strategy.