FIG. 2.

Results of molecular screening through a liver cDNA library. (A) Sets 61 and 191 of mixed cDNA clones were capable of supporting HCV replication (upper panel). The positive results of the HCV infection assay for two single cDNA clones (61.31 and 191.9) are shown (lower panel). C, cell lysate; M, the second aliquot of fresh medium used to wash the cells; S, HCV-positive serum. (B) Hygromycin B was removed from the medium to allow loss of episomal plasmid DNA. The HCV infection assay was performed every week (w) up to 7 weeks after removal of hygromycin B.