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. 2022 Feb 18;11(11):2102345. doi: 10.1002/adhm.202102345

Figure 4.

Figure 4

KRASG12D induces papillary neoplastic growth, dysplastic marker expression, and oncogene‐induced senescence. A) Schematic representation illustrating the propagation of pancreatic duct‐like organoids (PDLOs) harboring a Tet‐On expressing oncogenic HA‐tagged KRASG12D and mCherry on porcine urinary bladder. B) Immunohistochemistry stainings for HA‐tag and mCherry on vehicle and doxycycline‐treated Tet‐On expressing KRASG12D PDLOs cultured for four weeks on porcine urinary bladder (n = 4 for untreated (untr), n = 6 for doxycycline‐treated). C) Hematoxylin and eosin (H&E) stained histological sections of vehicle and doxycycline‐treated Tet‐On expressing KRASG12D PDLOs cultured for four weeks on porcine urinary bladder and contingency table comparing presence of papillary structures and doxycycline treatment (n = 4 for untreated, n = 6 for doxycycline‐treated). Immunohistochemistry stainings for D) CK‐19, E) CA19‐9, F) MUC1, G) MUC5AC, H) KI‐67, I) P53, and J) P21, and quantifications of respective positive cells in vehicle and doxycycline‐treated Tet‐On expressing KRASG12D PDLOs cultured for four weeks on porcine urinary bladder (n = 4 for untreated (untr), n = 6 for doxycycline‐treated). Data are shown as mean ± SD. *, p < 0.05, **, p < 0.01. Scale bars represent 100 µm. PSCs, pluripotent stem cells; PUB, porcine urinary bladder.