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. 1986 May 15;236(1):303–306. doi: 10.1042/bj2360303

Theoretical prediction and experimental measurement of the bile-pigment isomer pattern obtained from degradation of catalase haem.

N J Brindle, A C North, S B Brown
PMCID: PMC1146821  PMID: 3790079

Abstract

Degradation in vitro of the haem in catalase by a 'coupled oxidation' reaction yields products in which approx. 45% of the haem groups have been cleaved at the alpha-methene bridge, 55% at the beta-bridge and a trace at the delta-bridge. Molecular-mechanics calculations with the three-dimensional structural co-ordinates of catalase shows that these proportions of products can be accounted for by the relative accessibility of the four methene bridges to a haem-linked oxygen molecule, thus further confirming Brown's [(1976) Biochem. J. 159, 23-27] hypothesis that the first stage of haem catabolism in vivo is selective attack by haem-bound oxygen, with selectivity conferred by the surrounding protein moiety.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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