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. 2021 Aug 11;11(5):2101202. doi: 10.1002/adhm.202101202

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© 2021 The Authors. Advanced Healthcare Materials published by Wiley‐VCH GmbH

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Physicochemical characterization of SKOV3 EVs. A) Western blot analysis of EV protein markers (Alix, TSG101, CD81, CD9, and CD63) and EV‐negative markers (Calnexin) in SKOV3 cell lysate (CL) and SKOV3 EVs (EV). B) Size distribution of EVs as determined by NTA. C) Average diameter of EVs as determined by dynamic light scattering. D) Polydispersity index of EVs as measured by dynamic light scattering. E) Surface charge (zeta potential) of EVs as measured by laser Doppler electrophoresis. F) Cryo‐electron microscopy image of EVs isolated from SKOV3 cells. Data are shown as mean ± SD (n = 3, technical replicates).