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. 2022 Sep 15;11(22):2201877. doi: 10.1002/adhm.202201877

Figure 2.

Figure 2

Extraction and identification of microtia chondrocytes. Microtia chondrocytes were previously discovered to have both chondrocyte and stem cell‐like characteristics,[ 19 ] here immunofluorescence staining and q‐RT PCR detection were performed for the characterization of them. A) A diagram of a patient with microtia and the optical image of microtia tissue. B) Immunofluorescence staining of chondrogenesis‐related markers COL2 and Aggrecan of microtia chondrocytes (scale bar: 100 µm). C) Immunofluorescence staining of stem cell‐related markers including CD90, CD105, and CD73 of microtia chondrocytes (scale bar: 100 µm). D,E) mRNA expression analysis of chondrogenesis‐related genes including COL2A1, Aggrecan and Elastin, and stem cell‐related genes including OCT4A, NANOG and SOX2 in P1, P2 and P3 microtia chondrocytes (*p < 0.05, ***p < 0.001, **** p < 0.0001).