Figure 4.

F Cell‐instructive AP layers with distinct functional features for cultivation of HUVECs. A) Adhesion of HUVECs on RGDSP‐functionalized AP (10–30–8–2–RGD) layers, fibronectin (FN), or antiadhesive APs with increasing PEGylation and chain length 10–30–16–2 and 10–30–48–10. B) Quantification of cell numbers. C) Variation of RGDSP ligand density from 0 to 3,1% (2.8 pmol cm⁻²), 6,1% (5.6 pmol cm⁻²), 12.5% (11.3 pmol cm⁻²), 25% (22.6 pmol cm⁻²), 50% (45 pmol cm⁻²) as well as 100% RGDSP (90 pmol cm⁻²). D) Quantification of cell number versus ligand density. E) Switching of surface properties from adhesive to antiadhesive via targeted exchange of AP 10–30–8–2–RGD by AP 10–30–16–2; quantification of adhered cells before and after AP displacement analyzed after 24 or 72 h. F) Programming cell instructive signals by variation of ligand type and concentration by mixtures of 10–30–8–2–RGDSP, 10–30–16–2–HEP, and 10–30–48–10. G,H) Quantification of cell density (G) and of cell elongation (H) after 48 h on the AP‐coated surfaces displaying multiple ligands. Staining (A,C,F): nucleus: Hoechst 33342 (blue), actin: Atto‐555 Phalloidin (orange). Scale bars (A,C,F): 100 µm.